Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder) Calculate how to make a series of 1:10 dilutions of this stock. Imagine you’ll use 5 ml of each dilution for your experiment. Calculate the final concentrations of each tube tube 1: take some stock, dilute it 1:10 with some water in a new tube = #1 tube 2: take some of #1, dilute it 1:10 with some water in a new tube tube 3: take some of #2, dilute it 1:10 with some water in a new tube tube 4: take some of #3, dilute it 1:10 with some water in a new tube First, decide the total volume to make of each tube-keep in mind measurement accuracy (consider preparing 20% more solution than what you will use). Then, calculate how much stock, water and final concentration of each. Then, instead of doing a serial dilution, calculate making each of the above solutions using the weight/volume % solution method. Pros and Cons of serial dilution method vs W/V method
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